Shah and colleagues take advantage of the presence of maternal and somatic ribosomes in zebrafish and confirm their differential expression during development. The authors convincingly show that ...
The MCs were cultured in the DMEM medium of 5.5 mM glucose supplemented with 5% fetal bovine serum (FBS) (Invitrogen) at 37°C in a 5% CO 2 atmosphere. The sgRNAs of MCs were synthesized by ...
All cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; Sigma) and 1% penicillin/streptomycin (Gibco) at 37°C in a humidified chamber ...
supplemented with 50 U/ml penicillin and streptomycin (Invitrogen), 15% fetal calf serum (FBS, Invitrogen), 0.2 mM non-essential amino acids (NEAA, Invitrogen) and 2 mM glutamine (Invitrogen). For ...
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