Borden grew up one of four boys and two girls in a house on Sherman Avenue just west of the bridge over Buck Creek. The area was known as “The Bottoms” and decades earlier as “The Black Bottoms” for ...
over 10 minutes. The lysates were clarified by centrifugation at 30,000g for 20 minutes and proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific). The proteins were eluted with ...
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