Viral vectors expressing proneural genes can stimulate adult mammalian retinal glia to become immature neurons. These glia-derived neurons share properties with the bipolar, amacrine and retinal ...
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CAS Key Laboratory of Bio-Inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China Department of ...
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Briefly, the normal pancreas was dissected by manual mincing using a scalpel, followed by enzymatic digestion with 3.0 mg/mL collagenase A (Roche ... were quenched by adding DMEM containing 10% fetal ...
Various ocular cell types were plated at 1 × 10 4 cells/well of a 96-well tissue culture plate in 0.1 ml of their corresponding growth medium overnight. The next day, each well received their ...
Briefly, fresh tissues were homogenized in ice-chilled isolation buffer (1:10, w/v) using Dounce homogenizers and centrifuged at 1,000g for 5 min at 4°C. Supernatants were saved and transferred to an ...
The former relates to relapse-onset disease (although the appearance of MRI changes an extraordinary 10 years prior to clinical presentation of PPMS10 implies a preclinical phase that could represent ...