Next, we prepared a digestion solution by mixing a 0.1% trypsin (Sigma-Aldrich, T1426-500MG, St. Louis, MO, USA) with the 0.035 M PBS solution. The sample was then placed in this digestion solution at ...
The supernatant of positive fecal samples was first filtered through a 0.22-micron Millipore filter (Millipore Inc, USA), and then 10 micrograms per milliliter of N-tosyl-L-phenylalanine chloromethyl ...
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