This article describes the histone western blot protocol, which is often used at Abcam for identifying histone proteins obtained from purified calf thymus. For each lane, either 0.5 μg of calf ...

Histone methylation is an important epigenetic phenomenon that participates in a diverse array of cellular processes and has been found to be associated with cancer. Recent identification of ...

ChIP analysis was performed, followed by next-generation sequencing of histone methylation. Western blot, quantitative reverse-transcriptase PCR (qRT-PCR), and immunostaining were used to ...