Integration of these peaks gives the data in the bottom panel. The main objective of this analysis was to validate ITC for application in kinetic assays for this enzyme system, and compare ITC-derived ...

This article outlines how ITC provides real-time, enzyme kinetics data, in comparison to alternative enzyme assays. In part 2, how to perform enzyme kinetics investigations with a MicroCal ITC system ...

Measuring Enzyme Kinetic Parameters with the ITC Universal Enzyme Assay Method Enzymes are important in drug discovery and development since they constitute approximately one-half of all known ...

Real-time measurement of the initial stage of an enzyme–substrate reaction is essential for reproducible enzyme kinetics data. Automated direct-flow injection systems couple with various detection and ...

A library of 160 potential, in-house synthesised, elastase inhibitors were screened using a fluorescence assay with the internally quenched substrate Abz-Ala-Gly-Leu-Ala-Nba. Enzymatic kinetic ...

Drawbacks of Commonly Used Universal Kinase Assays Most commercially available kits have shortcomings that restrict their use in studying enzyme kinetics or for drug screening. Traditional kinase ...

Designed to quickly measure deubiquitinase activity or inhibitor screening in purified enzyme systems and cell-based models - these new assay kits come optimized for both kinetic and endpoint analysis ...

Colorimetric tests permit the indirect determination of the concentrations of specific substrates like carbohydrates or proteins and also the measurement of enzyme activity through change in color.