Ambrose is a city in Coffee County, Georgia, United States. As of the 2020 census, the city had a population of 327. Ambrose is located at 31°35…Ambrose is a city in Coffee County, Georgia, United States. As of the 2020 census, the city had a population of 327. Ambrose is located at 31°35′38″N 83°0′57″W / 31.59389°N 83.01583°W. According to the United States Census Bureau, the city has a total area of 3.1 square miles, of which 3.1 square miles is land and 0.1 square miles is water.